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Dade Behring diffquik staining kit
Diffquik Staining Kit, supplied by Dade Behring, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diffquik+staining+kit/diffquik+staining+kit/pm37696225-178-15-18
Average 90 stars, based on 1 article reviews
diffquik staining kit - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: [Ca 2+ ] i as a potential downregulator of ? 2 ? 1 -integrin-mediated A2058 tumor cell migration to type IV collagen
Article Snippet: .. The chamber assembly was placed into a 5% CO 2 37°C environment for 4 h. Upon completion of the experiment, the filter was stained with DiffQuik staining kit (Dade International, Miami, FL), and cells on the underside of the filter were visually counted under ×10 bright field magnification. .. In cases involving EGTA (Sigma), the chemotactic solution contained EGTA at 3.2 mM to chelate 1.6 mM Ca 2+ in basal medium.

Article Title: Absence of in vitro innate immunomodulation by insect-derived short proline-rich antimicrobial peptides points to direct antibacterial action in vivo.
Article Snippet: Some antimicrobial peptides (AMPs) have been described to exert immunomodulatory effects, which may contribute to their in vivo antibacterial activity.. Very recently, we could show that novel oncocin and apidaecin derivatives are potently antibacterially active in vivo.. Therefore, we studied oncocin and apidaecin derivatives for their effects on murine dendritic cells (DC) and macrophages and compared themwith well-known immunomodulatory activities of murine cathelicidin-related antimicrobial peptide (CRAMP).

Article Title: Use of Green Fluorescent Protein-Conjugated β-Actin as a Novel Molecular Marker for in Vitro Tumor Cell Chemotaxis Assay
Article Snippet: .. The chamber assembly was placed into a 5%CO 2 /37 °C environment for 4 h. The number of migrated cells was determined by staining filters using the DiffQuik staining kit (Dade International Inc., FL) and counting cells on the bottom side of the filter under 10× bright field magnification. ..

Article Title: Lifestyle and demographic factors associated with human semen quality and sperm function.
Article Snippet: In this study, we sought to investigate the effect of lifestyle and demographic factors on classic and functional semen parameters.. Three hundred and twenty-eight subjects who underwent semen analysis were recruited.. Routine SA, sperm vitality, acrosome reaction (AR) assay and sperm DNA fragmentation index (DFI) were analyzed.

Article Title: Melanoma cell migration to type IV collagen requires activation of NF- κ B
Article Snippet: .. The chamber assembly was placed into a 5% CO 2 /37°C environment for 4 h. The filter was stained with the DiffQuik Staining Kit (Dade International Inc., Miami, FL, USA) and cells on the underside of the filter were visually counted using a bright field microscope. ..

Article Title: Effects of COVID-19 or vaccines for SARS-COV-2 on sperm parameters: A systematic review and meta-analysis.
Article Snippet: This systematic review and meta-analysis assessed the effects of SARS-Cov-2 and mRNA vaccines on male reproduction.. We conducted a comprehensive search using terms such as "COVID-19," "vaccine," and "sperm parameters" on various electronic databases, including MEDLINE (PubMed), BVS (LILACS and others), Embase (Elsevier), and Cochrane Library.. We included observational studies, including cohort, case-control, and crosssectional studies.

Migration:

Article Title: Absence of in vitro innate immunomodulation by insect-derived short proline-rich antimicrobial peptides points to direct antibacterial action in vivo.
Article Snippet: Some antimicrobial peptides (AMPs) have been described to exert immunomodulatory effects, which may contribute to their in vivo antibacterial activity.. Very recently, we could show that novel oncocin and apidaecin derivatives are potently antibacterially active in vivo.. Therefore, we studied oncocin and apidaecin derivatives for their effects on murine dendritic cells (DC) and macrophages and compared themwith well-known immunomodulatory activities of murine cathelicidin-related antimicrobial peptide (CRAMP).

Incubation:

Article Title: Absence of in vitro innate immunomodulation by insect-derived short proline-rich antimicrobial peptides points to direct antibacterial action in vivo.
Article Snippet: Some antimicrobial peptides (AMPs) have been described to exert immunomodulatory effects, which may contribute to their in vivo antibacterial activity.. Very recently, we could show that novel oncocin and apidaecin derivatives are potently antibacterially active in vivo.. Therefore, we studied oncocin and apidaecin derivatives for their effects on murine dendritic cells (DC) and macrophages and compared themwith well-known immunomodulatory activities of murine cathelicidin-related antimicrobial peptide (CRAMP).

Article Title: Effect of antimicrobial peptides from Apis mellifera hemolymph and its optimized version Api88 on biological activities of human monocytes and mast cells.
Article Snippet: Apidaecin peptides are produced by the honeybee Apis mellifera as a major part of its non-specific defense system against infections.. Having verified that the peptides apidaecin 1b and Api88—a designer peptide based on the native apidaecin 1b sequence—are highly active against Gram-negative bacteria, we studied their ability to modulate biological activities of human monocytes and mast cells (MC), two important cell types of the human innate immune system.. We could show that both peptides are nontoxic and fairly resistant to degradation in cell culture medium containing 10% FBS.

Microscopy:

Article Title: Lifestyle and demographic factors associated with human semen quality and sperm function.
Article Snippet: In this study, we sought to investigate the effect of lifestyle and demographic factors on classic and functional semen parameters.. Three hundred and twenty-eight subjects who underwent semen analysis were recruited.. Routine SA, sperm vitality, acrosome reaction (AR) assay and sperm DNA fragmentation index (DFI) were analyzed.

Article Title: Melanoma cell migration to type IV collagen requires activation of NF- κ B
Article Snippet: .. The chamber assembly was placed into a 5% CO 2 /37°C environment for 4 h. The filter was stained with the DiffQuik Staining Kit (Dade International Inc., Miami, FL, USA) and cells on the underside of the filter were visually counted using a bright field microscope. ..



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Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
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Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with <t>DiffQuik</t> ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.
Diffquik Staining Kit, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diffquik+staining+kit/diffquik+kit/pmc06506732-46-9-12
Average 90 stars, based on 1 article reviews
diffquik staining kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

Journal: Cancers

Article Title: ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer

doi: 10.3390/cancers11101553

Figure Lengend Snippet: Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

Article Snippet: After 24 h, the membranes were fixed with 100% methanol and stained with a DiffQuik stain kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA).

Techniques: Expressing, Invasion Assay, Transfection, Plasmid Preparation, Staining, Microscopy, Incubation, Fluorescence